The US giant has been delaying delivery of printed books from Sweden's media and publishing group Bonnier, which has 16 publishers in Germany, since early May, the German Publishers' and Booksellers' Association said.
It claimed that Amazon's aim was to negotiate bigger price cuts on the purchase of e-books which the association put at up to 50 percent, a figure for which it cited media reports.
(Also see: Booksellers Score Some Points in Amazon's Standoff With Hachette)
Bonnier, which owns German publishing houses such as Ullstein and Carlsen, has not confirmed it is in any dispute with Amazon, nor the reported details.
"Amazon is breaching anti-trust law with its blackmailing approach to publishing houses," the association's head Alexander Skipis said in a statement.
"Amazon's business conduct doesn't just have consequences for the affected publishers, but represents a danger for all providers and distributors of e-books in Germany," he added.
The German competition watchdog must now decide whether to investigate the complaint.
For more than a year Amazon has been hit by intermittent strike action in Germany, its biggest market outside the United States, in a dispute over pay.
Amazon's employment practices were the subject of a critical documentary broadcast on German public television last year.
The German Publishers' and Booksellers' Association represents the interests of about 6,500 publishing companies, bookshops and booksellers.
For the latest tech news and reviews, follow Gadgets 360 on X, Facebook, WhatsApp, Threads and Google News. For the latest videos on gadgets and tech, subscribe to our YouTube channel. If you want to know everything about top influencers, follow our in-house Who'sThat360 on Instagram and YouTube.